cDNA微阵列技术对肺鳞癌、肺腺癌基因表达谱的研究

Non small cell lung cancer gene expression profiles by cDNA microarrays

目的 :利用cDNA微阵列技术研究肺鳞癌、肺腺癌基因表达谱 ,并与肺炎性假瘤基因表达谱进行比较 ,筛选肺鳞癌、肺腺癌的差异表达基因 ,及肺鳞癌、肺腺癌的共同表达基因 ,为研究肺癌的分子机制及肺癌的早期诊断和治疗提供线索。方法 :用含 4 0 96个基因的人基因表达谱芯片研究肺鳞癌、肺腺癌及肺炎性假瘤的基因表达谱。结果 :肺鳞癌差异表达基因 5 91个 ,其中筛选出在肺炎性假瘤中没有差异表达的基因 4 76个 ,上调的有 2 93个 ,下调的有 183个 ;肺腺癌差异表达基因 5 2 4个 ,其中在肺炎性假瘤中没有差异表达的基因 4 6 0个 ,上调的有 2 14个 ,下调有 2 4 6个 ;有113个基因在肺鳞癌与肺腺癌中均出现差异表达。结论 :肺鳞癌、肺腺癌存在不同的基因表达谱 ,筛选出的特异表达基因和共同表达基因为进一步研究肺癌的发生、发展和肺鳞癌与肺腺癌的分子特征提供了重要线索。

Objective To study lung squmamouscarcinoma and lung adenocarcinoma gene expression profiles by cDNA microarrays, compare them with those of pulmonary inflammatory pseudoneoplasma, and screen the differential expression gene and the common expression gene in these types of lung cancer in order to further study early diagnosis and treatment of lung cancer. Methods cDNA microarray chips containing 4096 human target genes were used to identify gene expression profiles in lung squamous cell carcinoma, adenocarcinoma, and pulmonary inflammatory pseudoneoplasma. Results Among the target genes, 591 differentially expressed genes were identified in lung squamous cell carcinoma; among them, there were 476 genes unexpressed in pulmonary inflammatory pseudoneoplasma; 293 of 476 differentially expressed genes were up regulated and the other 183 were down regulated. There were 524 differentially expressed genes in adenocarcinoma; among them, 460 genes were not expressed in pulmonary inflammatory pseudoneoplasma; 214 of the 460 differentially expressed genes were up regulated and 246 were down regulated. However, there were a total of 113 genes differentially expressed the both lung squamous cell carcinoma and adenocarcinoma. Conclusion There were distinct gene expression profiles among lung squmous cell carcinoma and adenocarcinoma. All those gene expression profiles and genes secreened out provide important clues for further investigation of carcinogenesis, invasion and metastasis of non small cell lung cancer and its molecular characteristics.