TrKA和p75神经营养因子受体在嗅神经母细胞瘤的表达

Co-expression of TrkA and p75 neurotrophin receptor in extracranial olfactory neuroblastoma cells

目的 :探讨TrKA和p75神经营养因子受体在嗅神经母细胞瘤的表达及其临床意义。方法 :应用免疫组化和双荧光标记法对 10例鼻腔嗅神经母细胞瘤及 5例正常鼻腔嗅黏膜及 5例鼻咽癌组织进行TrKA和p75神经营养因子受体检测。结果 :免疫组化检测发现 10例鼻腔嗅神经母细胞瘤TrKA和p75蛋白均呈阳性表达 ,5例正常鼻腔嗅黏膜及 5例鼻咽癌组织均为阴性表达 ;双荧光标记显示所有肿瘤组织中TrKA和p75蛋白均在同一细胞表达。结论 :TrKA和p75神经营养因子受体的过度表达可能与嗅神经母细胞瘤的发生和发展有关 ;

Objective To determine whether TrkA and p75 are expressed in olfactory neuroblastoma (ON) and whether the evaluation of their expression by immunohistochemistry is useful to predict the outcome of ON. Methods Ten cases of ON and 5 nasopharyngeal carcinoma and 5 normal olfactory neuroepithelial of nasal cavity were examined for the expression of TrkA and p75 neurotrophin receptors using immunohistochemistry and double fluorescence labeling. Results The 10 cases of ON showed positive immunohistochemical staining for both TrkA and p75. Double staining revealed that almost all TrkA immunoreactive ON cells also contained p75 immunoreactive. In contrast, no TrkA or p75 immunoreactivity was detected in nasopharyngeal cells and normal olfactory neuroepithelial cells. Conclusion Nerve growth factor may play a role in the generation of ON and staining of TrkA and p75 may assist diagnosis of ON.