铜绿假单胞菌体外粘附肠上皮细胞对其细胞膜生物特性的影响

Study of the effects of Pseudomonas aeruginosa adhesive on membrane of intestinal epithelial cells in vitro

目的 探索铜绿假单胞菌粘附肠上皮细胞后细胞膜生物特性的变化规律及可能的机制。方法 采用体外肠上皮细胞培养模型 ,研究绿脓杆菌粘附后对肠上皮细胞活力、细胞膜磷脂酶A2 、细胞内钙、细胞膜磷脂成分、膜流动性的影响。结果 细菌粘附后 3h肠上皮细胞活力明显下降 ,PLA2 活性增高 ,细胞内钙离子浓度增加 ,肠上皮细胞膜荧光偏振度、微粘度和分子排列有序性系数即出现增加 ,膜流动性降低 ,细胞膜磷脂含量降低 ,磷脂酰肌醇 (PI)、磷脂酰胆碱 (PC)含量逐渐降低。结论 绿脓杆菌粘附后肠上皮细胞胞内钙超载所导致的膜PLA2

Objective To explore the mechanism of intestinal epithelial cell membrane injury due to Pseudomonas aeruginosa adhesion. Methods The intestinal epithelial cells were cultured and adhered with Pseudomonas aeruginosa . The changes in the viability of the cells and the activity of membranous PLA 2, the calcium content of cell, contents of phospholipid and membrane fluidity were observed. Results At 3rd h after the adhesion of Pseudomonas aeruginosa , the viability of the intestinal epithelial cells decreased significantly, but the activity of membranous PLA 2, the calcium content of cell increased significantly; the contents of phospholipid(PL), phosphatidylinositol(PI) and phosphatidylcholine(PC) in cell membrane decreased gradually, but the membrane fluorescence polarization and microviscosity of intestinal epithelial cell membrane increased significantly. Conclusion The activation of PLA 2 and the degradation of phospholipid due to the overloading of calcium in intestinal epithelial cells after the adhesion of Pseudomonas aeruginosa to intestinal epithelial cells might be the fundamental factors to result in the reduction of membrane fluidity.