HPLC法同时测定胃痛欣颗粒剂中大黄酸、大黄素、大黄酚的含量

Simutaneous determination of the contents of rhein,emodin and chrysophanol in Weitongxin Granules by HPLC

目的建立胃痛欣颗粒剂中大黄酸、大黄素、大黄酚的含量测定方法。方法采用高效液相色谱法 ,AccusilC1 8(4 6mm× 2 5 0mm ,1 0 μm)柱 ,流动相为 :甲醇 水 冰醋酸 (80∶2 0∶1 ,V∶V∶V) ,检测波长为 2 5 4nm。结果大黄酸、大黄素、大黄酚分别在 0 0 3～ 0 4 8μg ,0 0 2 5～ 0 4 0 μg ,0 0 2 5～0 4 0 μg内与峰面积呈线性关系 ,平均回收率 (n =3 )分别为 98 3 % (RSD =1 4 3 % ) ,97 6% (RSD=1 84 ) ,98 7% (RSD =0 5 7% )。

Objective To develop a HPLC method for the determination of the contents of rhein,emodin and chrysophanol in the Weitonxin Granules.Methods The measurement conditions used were as follows: Accusil C 18( 4.6 mm× 250 mm)column; mobile phase: methanol-H 2O-acetic acid(80∶20∶1,V∶V∶V); flow rate: 1 mL/min; wavelength for rhein, emodin and chrysophanol showed good linear relation. The recovery rates for the rhein, emodin and chrysophanol were 98.3%(RSD=1.43%),97.6%(RSD=1.84%),98.7%(RSD=0.57%)respectively.The conditions of extraction and detemination were optimized.Conclusions The procedure is simple and rapid.The method can be used for evaluation of the crude drugs and their preparations.