摘要
采取健康公鸡血液,提取红细胞。以禽病原性大肠杆菌O78-166分离的LPS分别致敏新鲜红细胞和醛化红细胞,置4℃下保存。在致敏后1～8d,应用上述致敏红细胞分别测定6个O78-166免疫鸡阳性血清,以比较上述红细胞的敏感性和有效使用期。结果表明:以LPS致敏的新红细胞做定量试验较为精确,以LPS致敏的醛化红细胞做大肠杆菌定性试验较为快捷、方便。
The erythrocyte was separated from the healthy cock.The fresh RBC and the aldehydized RBC were sensitized by the LPS abstracted from avian E.coli O 78 -166separately,and kept at4degrees centigrade.These two sensitized red cell were used in the determination of the positive sera of chickens vaccinated by O 78 -166,in order to compare their sensitivity and validity.The result showed that:the fresh RBC is fit for the quantitative analysis of antibody of avian colibacilosis,and the aldehydized RBC is fit for the qualitative analysis.
出处
《四川畜牧兽医》
2003年第1期20-20,22,共2页
Sichuan Animal & Veterinary Sciences
