SD大鼠面突未分化外胚间充质细胞的体外培养

Culture of rat facial primordial undifferentiated ectomesenchymal cells

目的 建立SD大鼠面突未分化外胚间充质细胞的体外培养模型。方法 采用消化贴壁法培养SD大鼠外胚间充质细胞 ;用倒置显微镜观察细胞生长特性 ;用免疫组化法鉴定细胞来源。结果 细胞呈纤维样细胞生长 ,生长稳定。免疫组化染色 ,抗HNK -1和抗VIM为阳性 ,抗CK为阴性。结论 培养的细胞为外胚间充质细胞 。

Objective To develop a culturing model for the rat undifferentiated ectomesenchymal cells. Methods The rat ectomesenchymal cells were separated by trysin, their morphological characters were observed by stereomicroscopy, and their origination was examined immunohistochemically. Results The cultured ectomesenchymal cells appeared to be fibroblast-like cells. Anti-HNK-1 and Anti-Vimentin were positive by immunohistochemical stain, and Anti Cytokeratin was negative. Conclusion The cultured cells in this study were ectomesenchymal cell and may be used to investigate the development of tooth and jaw.