快杀灵与敌杀死对小鼠骨髓细胞增殖抑制和染色体诱变效应

EFFECTS OF CELL PROLIFERATION INHIBITION AND CHROMOSOME ABERRATIONS OF KUAISHALING AND DALTERMETHRIN ON MOUSE BONE MARROW

目的 :检测快杀灵和敌杀死对小鼠骨髓细胞的增殖抑制和染色体诱变效应。方法 :分别按受试药的1/4、1/2和1×LD50(快杀灵和敌杀死的LD50 分别为72.8mg/kg和26mg/kg)3个剂量分5次(每次间隔24h)经口灌胃给小鼠染毒 ,每次灌胃量为20ml/kg;用细胞遗传学方法检测骨髓的细胞增殖指数、染色体结构畸变率和畸变细胞率的变化。 结果 :与阴性对照组比较 ,2种农药的3个剂量都使骨髓的细胞增殖指数显著降低 ,快杀灵作用后骨髓细胞增殖指数为3.38±0.42～3.40±1.16(P<0.00001) ,敌杀死作用后骨髓细胞增殖指数为5.12±0.47～5.97±0.62(P<0.001～P<0.0001) ;快杀灵大剂量组以及敌杀死的3个剂量组各自的染色体畸变率、畸变细胞率均有显著性增高 ,快杀灵大剂量诱导的染色体畸变率和畸变细胞率分别为8.87±1.65(P<0.001)和7.21±2.10(P<0.01) ,敌杀死诱导的染色体畸变率和畸变细胞率分别是11.74±2.81～23.42±2.17(P<0.001～P<0.0001)和9.36±1.52～17.33±3.68(P<0.001～P<0.0001) ;敌杀死引起的染色体畸变类型比快杀灵的多而复杂。 结论 :快杀灵和敌杀死既能抑制骨髓细胞增殖 ,又能诱发染色体损伤。以LD50 为评价标准 ,快杀灵抑制骨髓细胞增殖的效应较强 。

Purpose: To detect the effects of cell proliferation inhibition and chromosome aberrations of Kuaishaling and Deltamethrin on mouse bone marrow. Methods:Two insectisides were administrated to mice at three different doses of 1 / 4, 1 / 2, 1×LD 50 (single doses of 1 / 20, 1 / 10 and 1 / 5×LD 50, the LD 50 of Kuaishaling and Deltamethrin were 72.8 mg / kg and 26 mg / kg) by gavage once a day for five days respectively, single gavaged volume was 20 ml / kg; the cell proliferation indices, the rates of chromosome structural aberrations and the rates of the cells with chromosome aberrations were detected by the methods of cytogenetics. Results: The cell proliferation indices of mouse bone marrow cells decreased significantly at all the three tested doses of these two insecticides as compared with the negative control group, the cell proliferation indices induced by Kuaishaling and Deltamethrin were 3.38±0.42～3.40±1.16 (P<0.000 01) and 5.12±0.47～5.97±0.62 (P<0.001～P<0.000 1), respectively; both the rates of chromosomal structural aberrations and the rates of the cells with chromosomal aberrations increased significantly at the high dose of Kuaishaling and the three tested doses of Deltamethrin, the rates of chromosomal structural aberrations and the rates of the cells with chromosomal aberrations induced by the high dose of Kuaishaling were 8.87±1.65(P<0.001) and 7.21±2.10(P<0.01), respectively, those induced by the three tested doses of Deltamethrin were 11.74±2.81～23.42±2.17(P<0.001～P<0.000 1) and 9.36±1.52～17.33±3.68(P<0.001～P<0.000 1), respectively; the chromosomal aberration types caused by Deltamethrin were more complex than by Kuaishaling. Conclusions: Kuaishaling and Deltamethrin can not only inhibit cell proliferation, but also cause chromosome damages. Moreover, using LD 50 as the evaluating criterion, the effect of cell proliferation inhibition caused by Kuaishaling is stronger, whereas the effect of chromosome aberrations caused by Deltamethrin is stronger.[