晚期血吸虫病巨脾型患者肝脏胶原病理研究

THE PATHOLOGICAL RESERCH ON HEPATIC COLLAGEN TYPE Ⅰ, Ⅲ AND Ⅳ IN PATIENTS WITH ADVANCED SCHISTOSOMIASIS JAPONICA WITH SPLENOMEGALY

目的 探讨晚期血吸虫病巨脾型患者肝脏胶原病理特征。 方法 采集实验组与对照组肝组织标本 ,对肝组织采用 HE染色 ,对肝胶原采用免疫组化 L SAB法染色 ,并用多媒体电脑图文分析系统对各型胶原定量。 结果 在汇管区和纤维隔 型胶原分布最多 ,在肝窦壁 型、 型和 型胶原均有明显增加 ,各型胶原量显著高于对照组。肝小叶结构基本正常 ,肝细胞无明显坏死 ,肝组织无明显炎症 ,但可见粗大纤维隔形成和肝细胞水样变。 结论 型、 型和 型胶原均参与了肝窦壁毛细血管化 ,是晚期血吸虫病患者肝功能衰竭的重要原因之一。

Objective To explore the pathological characteristic of hepatic collagen in patients with advanced schistdsomiasis japonica with splenomegaly(ASJS). Methods The liver specimens of experimental and control group were collected. The hepatic organization was stained with HE technique and the collagens were stained with immunohistocytochemical LSAB technique and quantified with multimedia colour computer imaging analysis system. Results The collagen type Ⅰ was chiefly distributed in portal areas and fibrous septum; In the sinusoid, the collagen type Ⅰ, Ⅲ and Ⅳ were observed and the levels of then were higher than that in normal controls; The hepatic lobule architecture was mostly normal and there wasnt marked liver cells necrosis and liver organization inflammation, but the thick fibrosis septum and swelling hepatic cells were observed. Conclusion The liver collagen type Ⅰ, Ⅲ and Ⅳ played a role in process of transforming liver blood sinus into capillary and it was one of reasons that breed liver failure in patients with ASJS. (For Fig. 1-7, to see back cover)