摘要
EDTA定钙法是目前测定发酵液中乳酸含量的主要方法。本文采用对照乳酸结合高效液相色谱法对该方法的测定条件进行了研究。结果表明,发酵液中的乳酸必须预先用过量的CaCO3中和,再用NaOH调节pH至12.0以上,以钙黄绿素为指示剂时,测定结果的重复性和回收率较好。HPLC法与EDTA定钙法测定结果比较,发现EDTA定钙法测定的乳酸含量偏大,但可通过适当校正用于生产过程检测。
EDTAtitration is a main method for th e determination of lactic acid in fermented broth.In this paper,factors influencing determination of lactic acid were studied with cont rol experiment and high performance liquid chromatography(HPLC).The results indicated that lactic a cid in fermented,broth must be neutr alized with excessive CaCO 3 before titration,then the pH value of solution was adju sted to exceed12.0with NaOH with calceinas indicator,a good reprodu cible result and recovery rate could be achieved.The levels obtained by HPLC in comparison with EDTA titration showed that EDTAwould yield excessive lactic acid data,but after calibration,EDTA method could still be used for quality control during lactic acid pro cessing.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第3期102-105,共4页
Food Science
基金
安徽省自然科学基金资助项目(01041302)
