褪黑激素调节免疫和改善睡眠作用的研究

Study on Effects of Melatonin on Enhancing Immunity and Improving Sleep Quality

本文主要通过经口给予褪黑激素,对小鼠调节免疫和改善睡眠的作用进行研究。调节免疫实验选用健康昆明种2月龄雌性二级小鼠,分设对照组、低剂量组(0.5mg/kg·bw)和高剂量组(1.0mg/kg·bw)。对照组灌喂蒸馏水,实验组给予不同剂量褪黑素28d后,测定各项免疫指标。改善睡眠实验选用BALB/c1月龄雄性二级小鼠,组别设立同调节免疫实验。对照组灌喂蒸馏水,实验组给予不同剂量褪黑素后测定各项睡眠指标。结果表明,与对照组比较,低剂量褪黑素能提高足跖肿胀厚度22.3%(p<0.001),NK细胞活性62.2%(p<0.05),血清溶血素水平17.1%(p<0.05),抗体生成细胞数量5.3%(p<0.05),碳廓清吞噬指数α15.9%(p<0.05),延长戊巴比妥钠诱导的小鼠睡眠时间23.4%(p<0.001),提高睡眠发生率4倍(p<0.01);高剂量褪黑素能提高足跖肿胀厚度33.3%(p<0.001),延长戊巴比妥钠诱导的小鼠睡眠时间38.7%(p<0.001),提高睡眠发生率3.5倍(p<0.05)。结果提示:低剂量褪黑激素具有提高免疫力的功能,低、高剂量的褪黑激素具有改善睡眠功能。

The purpose of the study is to investi gate the effects of melatonin on enhancing immunity.2-month -old female Kunming mice were s elected in regulating immunity tests.And in the improving sleep tests 1-month -old male BALB /c mice w ere selected.Mice were divided into control,low -dose(0.5mg /kg.·bw)and high -dose(1.0mg /kg·bw)groups.Control group was given distilled water,low -dose and high -dose groups were g iven different melatonin doses.The results showed that,compared with control group,low dose melaton in could improve the thickness of tum escence toe by 22.3%(p <0.001),NK cell activity 62.2%(p <0.05)and levels of HC 50 17.1%(p <0.05),the cell' s quantity of antibody formation(PFC)5.3%(p <0.05)and the carbon clearance indexα15.9%(p <0.05).It could also prolong the sleep time 23.4%(p <0.001)and improve the sleep rate 4times(p <0.01).High dose melatonin could improve the thickness of tumescence toe by 33.3%(p <0.001),prolong the sleep time 38.7%(p <0.001)and improve the sleep rate 3.5times(p <0.05).Conclusion:These results indivated that low dose melatonin had the function of enhancing immunity,while low and high dose melatonin could contribute to improve sleep quality.