肝癌细胞株中肿瘤特异性抗原MAGE、GAGE、BAGE基因表达的研究

Expression of MAGE, GAGE, and BAGE genes in human hepatocellular carcinoma

目的 研究MAGE、GAGE、BAGE基因在肝癌细胞株中的表达情况,评价这些肿瘤特异性抗原作为肿瘤分子标记以及肿瘤免疫治疗特异性靶位的可能性。 方法 用RT-PCR检测国内建株的肝癌细胞株SMMC-7721、QQY-7701、BEL-7402中MAGE-1、MAGE-3、GAGE1-8、GAGE1-2和BAGE基因mRNA的表达,以GAPDH基因作为检测内对照,并与非肿瘤肝穿组织比较。 结果 肝癌细胞株SMMC-7721表达MAGE-1和BAGE基因;QQY-7701表达MAGE-3和BAGE基因;BEL-7402表达MAGE-1和GAGE1-2基因;3株肝癌细胞至少表达其中一个基因。肝硬化病人肝穿刺组织中MAGE、GAGE、BAGE基因表达均为阴性。 结论 MAGE、GAGE、B A G E肿瘤特异性抗原可以作为肝癌早期诊断的分子标记,并具有作为肝癌免疫治疗特异性靶位的潜在价值。

Objective To observe the expression of MAGE, GAGE and B AGE genes in human hepatocellular carcinoma (HCC) cell lines. Methods The expression levels of MAGE-1, MAGE-3, GAGE1-8, GAGE1-2 and BAGE mRNAs in HCC cell lines SMMC-7721, QQY-7701, BEL-7402 were studied by reverse transcription polymerase chain reaction and were compared with those in biopsied liver tissues. Results MAGE-1 and BAGE mRNAs were expressed in SMMC-7721 cells.MAGE-3 and BAGE mRNAs were expressed in QQY-7701 cells. MAGE-1 and GAGE1-2 mRNAs were expressed in BEL-7402 cells. None of these genes was expressed in biopsied liver tissues. Conclusions MAGE-1, MAGE-3, GAGE1-8, GAGE1-2 and BAGE are expressed in hepatocellular carcinoma cell lines. These tumor-specific antigens can be used as molecular markers for early diagnosis and possible targets for immunotherapy of human HCC.