重型乙型肝炎外周血单核细胞基因表达谱研究

Gene expression profile of peripheral blood monocyte in patients with fulminant hepatitis B by cDNA microarray

目的 应用基因芯片技术建立慢性重型乙型肝炎外周血单核细胞基因表达谱。 方法 应用含8192条人体cDNA的微阵列芯片和来自外周血单核细胞的标记cDNA,分析了10例慢性重型乙型肝炎和10例无症状HBsAg携带者(ASC)基因表达谱。通过应用GenePix 4000B扫描仪和ImaGene3.0分析软件比较Cy5标记的慢性重型肝炎来源cDNA与cy3标记的ASC来源cDNA的杂交结果,获得个体基因的相对表达比值。 结果 在8192个基因中,初筛出249个(3％)表达差异达2倍以上的基因,其中主要是细胞信号和传递,细胞周期和代谢,凋亡及炎症相关类基因(占79％)。 结论 这些结果提示了乙型肝炎病毒感染机体致慢性重型肝炎过程中,全面改变了宿主细胞内基因表达,并为进一步对这些差异表达基因的功能研究提供了一个框架。

Objectives To investigate peripheral blood monocyte (PBMC) gene expression profile in patients with fulminant hepatic failure (FHF) by cDNA microarray. Methods Microarrays consisting of 8 192 human cDNAs and labelled cDNAs prepared from PBMC in both 10 FHF patients and 10 asymtomatic surface antigen carriers (ASC) were applied to analyze gene expression. Relative ratios of gene expression in individuals were obtained by comparing the hybridization results, by GenePix 4000B scanning and by ImaGene3.0 software analysis, of Cy5-labelled cDNA from FHF patients with those of Cy3-labelled cDNA from ASC. Results 249 genes out of 8 192 were identified differently, at least two times. Most of the genes (79%) involved in cell signaling transduction, cell cycles, metabolism, inflammatory response and apoptosis, whose mRNAs were differently regulated. Conclusions These results suggest that HBV infection alters a broad range of cellular genes expression during developing into FHF and provide a framework for future functional study on the genes expressed differently.