声表面波免疫传感器的设计及应用

The design and application of surface acoustic wave immunosensor

声表面波免疫传感器是一种新型生物免疫传感器。它利用声表面波振动的敏感性,同时结合了免疫反应的特异性,作为免疫检测的手段,可以对多种抗原或抗体进行快速的定量测定,具有高特异性、高灵敏度、响应快、小型简便等特点。本文利用声表面波延迟线振荡器作为传感器,检测溶液中人体免疫球蛋白IgG的含量。实验采用双通道系统进行频率变化的测定,并分别采用氨基硅烷膜,蛋白A两种方法将抗体固定到反应区域表面。根据实验结果,我们得到了频率变化和质量附着的定量关系,并与理论分析的结果相比较。

Surface acoustic wave (SAW) immunosensor is a new type of biosensor. By using the sensitivity of SAW vibration and the specific binding ability of immune reaction, the sensor can be developed for rapid quantitative analysis of various antigens and antibodies, providing properties of high specificity and sensitivity, rapid response, minitype and simplicity.In this paper, a SAW delay-line oscillator is used to detect the quantity of human immunoglobulin G in a buffer. The setup utilizes a dual-channel system to detect the relevant frequency shift, and we immobilize the antibody onto the reaction surface region with two methods, γ-aminopropyltriethoxysilane and protein A. From the experimental results, we derive a quantitative relation between the frequency shift and the mass loading, and compare it with the result of theoretical analysis.