鸡血藤活性成分SS8对骨髓抑制小鼠造血祖细胞增殖的作用

Effect of SS8, the Active Part of Spatholobus suberectus Dunn, on Proliferation of Hematopoietic Progenitor Cells in Mice with Bone Marrow Depression

目的:研究鸡血藤单体成分SS8对骨髓抑制小鼠造血祖细胞(CFU-GM,CFU-E,BFU-E,CFU-Meg)增殖的影响。方法:采用甲基纤维素半固体培养法,通过对长期接受SS8治疗后的骨髓抑制小鼠造血祖细胞的培养,观察SS8对CFU-GM,CFU-E,BFU-E,CFU-Meg生长的作用。结果:SS8可显著刺激骨髓抑制小鼠CFU-GM,CFU-E,BFU-E,CFU-Meg生长,与对照组比较有显著差异(P<0.05)。随时间延长、剂量增加,刺激作用逐渐加强。结论:SS8对骨髓抑制小鼠造血祖细胞的增殖有明显刺激作用,且有时间、剂量相关性。

Objective: To investigate the effect of SS8, a monomer extracted from Spatholobus suberectus Dunn used for invigorating the circulation of blood , on proliferation of hematopoietic progenitor cells in mice with bone marrow depression. Method: The method of semi-solid culture with methylcellulose of CFU-GM, CFU-E, BFU-E, CFU-Meg was adopted in bone marrow depressed mice which were treated with SS8 for a long time. Result: The experimental data demonstrated that the numbers of CFU-GM, CFU-E, BFU-E, CFU-Meg in bone marrow depressed mice were raised distinctly under the control of SS8 as compared with those of contrast group. The effect of SS8 became stronger as time went on and the dosage rose. Conclusion: SS8 can distinctly stimulate the proliferation of hematopoietec progenitor cells in mice with bone marrow depression in a time-and-dosagedependent manner.