不同产地红花药材的高效毛细管电泳指纹图谱研究

Study on the fingerprints of Flos Carthami from different sources by HPCE

目的 建立红花道地药材的高效毛细管电泳指纹图谱 ,并对不同产地红花药材的指纹图谱进行比较。方法 以 60 %甲醇溶液提取 ,采用毛细管区带电泳法 ,运行电压 2 4kV ,运行缓冲液为 5 0mmol·L-1硼砂 ( pH 9.7)其中含 18%甲醇 ,以利福平为参照物 (IS) ,建立了 10批红花道地药材的指纹图谱 ,并与 9个产地的红花药材以及红花的对照药材加以比较。结果 在红花道地药材的指纹图谱中 ,共有峰的相对迁移时间的RSD <1.8% ,相对峰面积的RSD <6.2 % (n =5 )。不同产地红花药材的指纹图谱中 ,共有峰的相对峰面积有一定的差别。结论 HPCE指纹图谱分析法可作为红花药材的质量控制方法。

OBJECTIVE: To develop the fingerprint of Flos Carthami by high performance capillary electrophoresis (HPCE) and compare the fingerprints of 10 samples from different sources. METHODS: Aqueous methanol of 60 % was used as the extract solvent and capillary zone electrophoresis (CZE) was used to determine the fingerprints. The applied voltage was 24 kV and the detection wavelength was 210 nm. The buffer was composed of 50 mmol&middotL-1 borate solution (pH 9.7) containing 18 % methanol and rifampicin was used as a reference standard. The fingerprints of 10 batches of Flos Carthami from the place of the genuine were studied and the fingerprints of the samples from 9 different sources were compared. RESULTS: The RSD of the relative migration times was below 1.8% and that of the relative peak areas was within 6.2% (n = 5). Regarding to the fingerprints of the samples from different sources, there were differences in the relative areas of common peaks. CONCLUSION: HPCE can be used as the identification method for the Flos Carthami.