灯盏花制剂对大鼠缺血性脑损伤再灌注早期脑成像及神经代谢物的作用

The Effects of Erigeron breviscapus Preparation on the Imaging and Neuronal Metabolites at the Early Time Points After Reperfusion Following the Ischemic Cerebral Injury in Rats

目的:研究灯盏花制剂对缺血性脑损伤脑成像的影响及脑组织代谢物的作用。方法:雄性SD大鼠23只,随机分成缺血损伤组(n=11)和灯盏花治疗组(n=12),分别用于核磁共振和大脑表面血管的观察。颈部正中切口,线栓法造成右大脑中动脉血供阻断(MCAO)。缺血2h后,立即腹腔注射灯盏花注射液(1.5mg·kg-1体重)。采用核磁共振T2加权成像(T2WI)和局域质子谱(1H-MRS)技术,研究大脑成像和神经元代谢物N-乙酰天门冬氨酸(NAA)、肌酸/磷酸肌酸(Cr/PCr)、胆碱(Cho)和乳酸(Lac)的变化。结果:灯盏花治疗组的高信号强度区信号较同时刻同时间点缺血损伤组减弱、体积小,T2值减小,NAA值大,Cr/NAA和Cho/NAA比值小,未见乳酸峰;大脑表面的血管呈扩张状态。结论:灯盏花有助于缺血脑组织的血液循环重建,改善神经元的代谢。

Objective:To investigate the effects of Erigeron breviscapus preparation on the imaging and neuronal metabolites after reperfusion in the ischemic cerebral injury in rats. Method: Twenty-three male Sprague Dawley rats with an average body weight of (165±15) g(mean±S) were used, and were randomly divided into two groups: group A rats(n=11) underwent an operation of ischemic brain injury, group B rats (n=12) underwent the same operation and received the treatment of Erigeron breviscapus preparation (1.5mg·kg-1 weight, ip). The right middle cerebral artery occlusion (MCAO) of rats in both groups was induced by 5/ 0 nylon suture for 2 hours. The reperfusion was conducted for four hours and six hours respectively following MCAO. T2 weighted-imaging (T2WI) and 1H-magnetic resonance spectroscopy (1H-MRS) were performed, to study the changes of the imaging and the neuronal metabolites N-acetyl aspartate (NAA), creatine/phosphocreatine (Cr/PCr), choline (Cho) and lactose (Lac) in cerebrum following cerebral ischemia. Result:The hyperintense signals in the right cerebrum in group B decreased compared with those in group A, the T2 values decreased, the level of NAA increased, the ratio of Cr/NAA and Cho/NAA decreased, and no lactose was observed. The brain surface vessels of rats in group B were in the state of dilation. Conclusion: Erigeron breviscapus preparation is beneficial to the reestablishment of the blood circulation in the ischemic brain, and to the improvement of the neuronal metabolism and survival.