EMP-1基因在喉癌中的表达差异分析

Differential Expression of the Epithelial Membrane Protein1of Laryngeal Carcinoma

目的分析上皮膜蛋白-1(epithelialmembraneprotein-1,EMP-1)基因在喉癌中的表达差异,以进一步探讨喉癌的发病机制。方法用微阵列分析技术分析喉癌基因的差异表达情况,选取其中之一的EMP-1基因,用半定量逆转录酶-聚合酶链反应(reversetranscriptasepolymerasechainreaction,RT-PCR)技术分析EMP-1基因在不同类型喉癌中的表达情况,数据分析采用SPSS软件。结果EMP-1基因在不同类型配对喉癌中皆呈低表达,明显低于相应的正常喉粘膜组织(P<0.05),EMP-1基因表达量与喉癌的分化类型、分期无明显关系(P>0.05)。结论EMP-1基因与喉癌的发病明显相关,揭示这种关系将有助于探讨喉癌的发病机制。

Objective In order to explore pathogenic mechanism of laryngeal carcinoma,the involved genes were identified in larynx carcinogenesis by comparing the gene expression profile in matched primary normal epithelial cells and primary laryngeal carcinoma cells from the same patients.Methods A cDNA microarray analysis consisting of11431human genes revealed signi -ficant changes in the expression of35genes,with8genes being up-regulated and27being down-regulated.The epithelial membrane protein-1(EMP -1)is one of the down-regulated genes.EMP -1expression in various kinds of laryngeal carcinoma was determined by semi -quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).Results The EMP -1mRNA levels in all laryngeal carcinoma cells was significantly lower than that in the matched primary normal epithelial cells(P<0.05)and were not correlated to the stage and differentiation of laryngeal carcinoma (P>0.05).Conclusions The EMP -1expression was correlated to larynx carcinogenesis and may be helpful to elucidate the pathogenic mechanism in laryngeal carcinoma.