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噬菌体表面呈现技术及在疫苗研制中的应用 被引量：3

Phage Display Technique and Application in Vaccine Research
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摘要 噬菌体表呈现展示技术 (PhageDisplayTechniques ,PDT)是将编码外源肽或抗体可变区的DNA片段插入噬菌体表面特定蛋白基因中 ,以融合形式与噬菌体的表面蛋白共同表达于噬菌体表面的技术 ,它将基因型与表型、分子结合活性与噬菌体的可扩增性结合在一起 ,是一种高效的筛选系统 ,因而在抗原表位的模拟、蛋白质工程的改造、单克隆抗体的制备、药物筛选及疫苗研制等方面 。 The phage display technique is inserted the coding exogenous peptide or alternative DNA segment of antibody into the specific protein gene on bacteriophage surface and express on the surface with confluenced with surface protein. It is a high effective screen system with combination of gene, phenotype, molecular activity and bacteriophage amplification. It had been widely used in antigen epitope modelling, protein engineering improvement, preparation of monoclone antibody, drug screening and study on vaccine.
作者 闫守庆 张玉静
机构地区 中国人民解放军军需大学
出处 《黑龙江医学》 2003年第2期110-112,共3页 Heilongjiang Medical Journal
关键词 医学微生物学 病菌噬菌体表面呈现 肽库 抗体库 Phage display Peptide library Antibody library
分类号 R37 [医药卫生—病原生物学]
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参考文献17

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同被引文献28

  • 1陶艳琳,程训佳.微小隐孢子虫致病相关蛋白的研究进展[J].中国寄生虫学与寄生虫病杂志,2004,22(4):253-255. 被引量：4
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引证文献3

二级引证文献6

黑龙江医学
2003年 第2期

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