气管滴入及腹腔注入内毒素致大鼠急性肺损伤机制的研究

The difference in acute lung injury caused by intratracheal instillation or intraperitoneal injection of LPS

目的 :探讨肺内因素及肺外因素致急性肺损伤 (ALI)的异同。方法 :气道内滴注及腹腔内注射内毒素(LPS ,10mg kg)建立大鼠ALI模型。 5 4只雄性SD大鼠随机分为对照组、气道组、腹腔组 ,每组 18只 (2h、6h、2 4h各6只 )。按时相处死并采集标本 ,观察大鼠动脉血气分析、支气管肺泡灌洗液 (BALF)总蛋白水平、细胞总数、细胞分类及TNF α水平、肺病理。结果 :和腹腔组比较 ,气道组大鼠动脉血氧分压 (PaO2 )明显降低 (P <0 0 0 1) ,BALF总蛋白水平、PMN明显增加 (P <0 0 0 1) ,BALF中TNF α水平明显升高 (2h、6hP <0 0 0 1,2 4hP <0 0 5 ) ,肺病理示肺内PMN大量浸润伴出血、透明膜形成。结论 :①气道内滴注LPS致大鼠PaO2 进行性降低 ,而腹腔内注射LPS对PaO2 无明显影响。②气道内滴注LPS致大鼠肺内PMN大量浸润 。

Objective:To investigate the difference in acute lung injury caused by pulmonary and extrapulmonary factor Methods: A rat model of ALI was induced by intratracheal(IT) instillation or intraperitoneal(IP) injection of LPS with a dose of 10mg/kg 54 male SD rats were randomly divided into control group?IT group and IP group,every group included 18 rats(6 rats at 2h?6h and 24h respectively) the arterial gas analysis?the total protein concentration?the total cell counts and classification?the level of TNF α in BALF?lung pathology of rats were examined Results: Comparision with IP group,in ITgroup the PaO 2 was decreased sharply( P <0 001), the total protein concentration?the total PMN in BALF?the level of TNF αin BALF were greatly increased( P <0 001or P <0 05), lung pathology showed severe polymorphonuclears infiltration with bleeding and hyaline membranes Conclusion: ①PaO 2 of rats in IT group decreased progressively,intraperitoneal injection of LPS has no effcct on PaO 2 of rats ②Overwhelming PMN infiltration was found in rats of IT group and was more severe than IP group