复发性尿路上皮肿瘤的克隆起源

Clone analysis on recurrence of urothelial carcinoma

目的 :判断复发性尿路上皮肿瘤的克隆起源。方法 :以聚合酶链反应 -单链构象多态性分析法 (PCR SSCP)和免疫组化染色的方法 ,分别检测了 2 0例上尿路上皮肿瘤和相应的复发性肿瘤p5 3基因的突变 ,以及 30例80块标本中的p5 3蛋白表达 ,比较原发和复发性肿瘤的基因型。结果 :复发性肿瘤和原发性肿瘤p5 3基因型一致者为 6 3 6 % ,不一致者为 36 4 % ,提示复发灶单克隆起源者约为 2 3,多克隆起源者约为 1 3。结论 :复发性尿路上皮癌多为单克隆起源 。

Objective:To investigate the clonal origin of malignant cell in recurrence of urothelial carcinoma Methods: The p53 gene mutations were evaluated by polymerase chain reaction single strand conformation polymorphism(PCR SSCP), and protein expressions were detected by immunohistochemistry in 30 patients with urothelial neoplasms and their corresponding recurrented 80 pieces of tumor tissues Mutations in p53 inferred on the basis of immunostaining Results: The p53 protein expression of recurrented tumor in identical patient completely matched was seen in 63 6%, and mismatched 36 4% The results indicated that two thirds of recurrented tumor are derived from the monoclonal cell, and one third is polyclonal Conclusion: The most of relapsed urothelial tumors are origined from monoclonal cell, and small part from polyclone