GABA对大鼠海马脑片缺氧损伤的保护作用

PROTECTIVE EFFECT OF GABA AGAINST HYPOXIC INJURY IN RAT HIPPOCAMPAL SLICES

目的 :研究GABA对大鼠海马脑片急性缺氧损伤的保护机制。方法 :采用成年大鼠离体海马脑片 ,用胞外记录的电生理技术 ,观察GABA对急性缺氧后海马脑片诱发电位的影响。结果 :①GABA可明显延迟PV的消失 ,但对PS却无影响 ;②给予GABAA 受体拮抗剂荷包牡丹碱 (bicuculine)以及Cl 通道阻抗剂NPPB可阻断GABA的保护作用。结论 :GABA可提高海马脑片耐缺氧妮力 ,其机制可能与GABA通过GABAA 受体提高Cl

Aim: To study the mechanism of protective effect of GABA against hypoxic injury in rat hippocampal slices. Methods: The hippocampal slices from adult rats and extracellular recording technique were used to observe the effect of GABA on the evoked population spikes in rat hippocampal slices after hypoxia in vitro . Results: GABA can significantly delay the disappearance of PV,but have no effect on PS. When the receptor antagonist of GABA(bicuculine) and the inhibitor of Cl - cannel(NPPB) were given,the protect effect could be suppressed. Conclusion: GABA increases hypoxic tolerance of hippocampal slices. The mechanism of the effect of GABA may be involved in the elevation of chloride influx through GABA receptor.