低温保存许旺细胞对周围神经再生的作用

EFFECT OF THE CRYOPRESERVED SCHWANN CELLS ON THE PERIPHERAL NERVE REGENERATION

目的 :比较原代培养许旺细胞 (Schwanncells ,SCs)和冷冻保存的SCs移植对损伤后坐骨神经再生的作用。方法 :原代培养和液氮保存的SCs分别移植到桥接缺损坐骨神经的硅胶管内。在移植后不同时间 (第 6和 8周末 ) ,硅胶管远端神经干内注射HRP ,逆行追踪背根神经节和脊髓前角的标记神经元数量 ;测量再生神经纤维的复合动作电位传导速度 ;电镜观察再生神经纤维的髓鞘形成。结果 :原代培养和冷冻保存SCs在移植后不同时间其背根神经节和脊髓前角神经元HRP标记细胞数量、再生神经纤维的复合动作电位传导速度基本一致 ,再生神经纤维髓鞘的形成未见明显差别。结论

Aim: To compare the effects of primary culture Schwann cells and cryopreserved Schwann cells on the injured peripheral nerve regeneration. Methods: The primary culture Schwann cells and cryopreserved Schwann cells were transplanted into the silicone tubes that bridged the resected sciatic nerves. At different time after transplantation, HRP was injected into the sciatic nerve trunks at the distal end of the silicone tubes, and then the HRP labeled neurons in dorsal root ganglions and anterior horns of spinal cord were counted. The complex action potential of regenerated nerve fibers was measured. Myelination on regenerated nerve fibers was investigated under electronic microscope. Results: At different time after transplantation, there were not significant differences that the HRP labeled neurons in dorsal root ganglions and anterior horns of spinal cord, the complex action potential of regenerated nerve fibers and myelination on regenerated nerve fibers ( P >0.05), between primary culture and cryopreserved Schwann cells. Conclusion: The cryopreserved Schwann cells still play the same important role in the regeneration of injured peripheral nerves as the primary culture Schwann cells do.