不同源性大肠杆菌多药耐药基因AcrA、AcrB部分基因的同源性分析

Homology Analysis of AcrA and AcrB Gene of Multiple Drug Resistance of Different Escherichia coli

通过聚合酶链式反应从不同源性大肠杆菌的染色体DNA上扩增出大肠杆菌多药耐药基因AcrA、AcrB的部分片段 ,将该片段分别连接到克隆载体pMD_18T的BamHI、XhoI酶切位点中 ,测定插入片段的核苷酸序列 ,并分别推导出其氨基酸序列。序列分析表明 :不同源性大肠杆菌的AcrA的部分基因的核苷酸序列及所推导的氨基酸序列与GeneBank中该基因序列的同源性较高 ;不同源性大肠杆菌的AcrB的部分基因的核苷酸序列的同源性较低 。

The part of AcrA and AcrB gene of multiple drug resistance of Escherichia coli were amplified by polymerase chain reaction(PCR) from DNA of Escherichia coli and fragments were ligated with the vector pMD_18T cut with BamH I and Xho I.Homology analysis of the part of AcrA and AcrB genes nucleotides sequence and deduced amino acids sequence revealed that the part of AcrA of different Escherichia coli had most similarity with that gene of GenBank,nucleotides sequence of the part of AcrB of different Escherichia coli had less similarity with that gene of GenBank,deduced amino acids sequence of the part of AcrB of different Escherichia coli had most similarity with that gene of GenBank.