七彩山鸡新城疫病毒的分离及生物学特性测定

Isolation and Biological Properties of Newcastle Disease Virus from Pheasant

用SPF鸡胚从疑似七彩山鸡新城疫病鸡群中分离到一株病毒 ,经HA及HI试验以及病毒中和试验结果表明 ,该分离毒为七彩山鸡新城疫病毒。动物回归试验表明 ,分离毒肌肉注射非免疫七彩山鸡能使之发病、死亡 ,出现与自然病例一致的症状与病变 ,但肌注SPF鸡只感染 ,不出现临床症状。其生物学特性为 :NHAT为快、HOT为 8分钟、ICPI为1.4 6、IVPI为 1.6、MDT为 76 .

One strain was isolated from pheasant which supposed to be infected with newcastle disease through SPF chicken eggs.The results indicated that the isolated was pheasant ND virus by hemagglutination test(HA),hemagglutination in hibition test(HI)and virus neutra lization test.The biological properties of the virus was determined by measuring the mean death time of chicken embryos(MDT),the intracerebal pathogenicity index(ICPI) and the intravenous pathogenicity index(IVPI).The MDT,ICPI and IVPI were 76.8,1.46 and 1.6 hours respectively.