摘要
根据已测得的卡氏住白虫ITS_2序列 ,在 3’端设计一种特异引物 ,另外采用一个位于 5’端的保守引物 ,建立了鸡卡氏住白虫病的PCR诊断方法。特异性试验和敏感性试验表明 ,该诊断方法与其他虫体如疟原虫、弓形虫、微孢子虫、球虫等无交叉反应性 ,能检测的卡氏住白虫最低DNA浓度为 10 0 0fg 。
Based on the ITS_2 rDNA sequence of Leucocytozoon caulleryi,which was obtained by Xue Hong,a species_specific primer(H8)were designed,together with a universal primer(H7),a diagnostic PCR assay for leucocytozoonsis was established.The special test and sensitive test showed that there was no cross reaction between L.caulleryi and other parasites,such as Palsmodium sp,Toxoplasma gondii,Eimeria tenella and Microspora sp.etc,and the lowest DNA concentration of L.caulleryi that could be detected was 100fg.This assay could be used as an earlier diagnostic assay for L.caulleryi infection.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2003年第2期148-151,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金资助项目 (39870 5 4 9)
广东省自然科学基金资助项目 (980 134)
关键词
卡氏住白虫
PCR诊断
特异性试验
敏感性试验
Leucocytozoon caulleryi
A diagnostic PCR assay
Special test
Sensitive test
