中国彝族人群HLA-Cw位点基因多态性研究

Genotyping of HLA-Cw locus in Chinese Yi ethnic group by PCR-SSP

目的 应用DNA分型技术 ,分析中国彝族人群在HLA Cw位点的等位基因分布频率 ,为进一步研究HLA Cw与HIV感染及其发病进程的关联提供背景资料。方法 设计合成 2 6条特异性分型引物和 1对内对照引物 ,组成 2 4个PCR反应 ,建立PCR SSP分型方法 ,对 10 2例随机选取的无亲缘关系的中国彝族健康人作HLA Cw基因分型。结果 在彝族健康人群中共检测到HLA Cw位点的12种等位基因 ,其中HLA Cw 0 1、Cw 0 7和Cw 0 8为主要分布型别 ,基因频率分别为 0 .3 3 3 3、0 .2 5 0 0和 0 .1765 ,检出了HLA Cw 12、Cw 13 0 1、Cw 14和Cw 15等血清学方法不能鉴定的HLA Cw基因 ;统计分析表明HLA Cw位点的基因型分布符合Hardy Weinburg平衡 (χ2 =65 .983 1,df=66,P >0 .0 5 )。结论 证实了PCR SSP法用于HLA Cw基因分型的可靠性和适用性 ,在DNA水平上提供了中国彝族群体的HLA

Objective To analyze the genetic polymorphism of HLA Cw locus in Chinese Yi ethnic group by DNA typing for further study on its association with HIV infection and progression to AIDS. Methods A rapid genotyping method for HLA Cw by PCR SSP was set up. It combined twenty six specific primers and one pair of internal control primer to form twenty four one step reactions for each sample. Totally 102 unrelated healthy Chinese Yi ethnic individuals were typed. Results Twelve HLA Cw alleles were detected in Chinese Yi ethnic group with HLA Cw *01, Cw *07 and Cw *08 as the most common genes, which accounted for a frequency of 0 333 3, 0.250 0 and 0.176 5 respectively; four kinds of non serologically defined HLA Cw genes i.e. Cw *12, Cw *1301, Cw *14 and Cw *15 were found in this population. Hardy Weinburg test showed that the genotype distribution observed was correspondent with the expected (χ 2=65.983 1, df=66, P >0.05). Conclusion This study provides the data of HLA Cw gene frequency in Chinese Yi ethnic group, which may contribute to research on anthropology, disease association and vaccine application. The result also confirmed that PCR SSP was a reliable and fast method for HLA Cw genotyping.