丙型肝炎病毒体外感染人肝癌细胞株HepG_2的研究

In vitro infection of human liver cancer cell line HepG_2 with HCV

目的 研究人肝癌细胞株HepG2 体外对丙型肝炎病毒 (hepatitisCvirus ,HCV)的易感性。方法 慢性丙型肝炎患者的血清与HepG2 共同孵育后 ,以逆转录 聚合酶链反应、免疫组化法、原位杂交法分别检测细胞和 (或 )培养上清中的HCV正、负链RNA、HCV抗原表达和HCVRNA在细胞内的定位。结果 感染血清和细胞共同孵育后的第 2～ 40天 ,从细胞内和培养上清中均可间断地检测出HCV正、负链HCVRNA ,HCVNS3抗原在细胞内能稳定表达 ,原位杂交证实HCVRNA阳性物质多位于细胞质中。结论 HepG2 细胞在体外不但对HCV易感 。

Objective To test susceptibility of human liver cell line Hep G 2 to HCV in vitro. Methods Hep G 2 was cultivated with the serum from a chronic hepatitis C patient.After inoculation,plus and minus strand of HCV RNA,the expression of HCV NS3 antigen and the location of HCV RNA in cell and/or supernatant were examined by RT PCR, immunohistochemistry and in situ hybridization,respectively.Results HCV RNA could be detected from day 2 to day 40 post inoculation in both cell and supernatant.HCV NS3 antigen could be expressed in infected cells and HCV RNA was mainly situated within cytoplasm.Conclusion The results suggested that HepG 2 cell line was not only susceptible to HCV but also could support its replication in vitro.