毛细管电泳法分析贝类食品中的软骨藻酸

Analysis of Domoic Acid in Shellfish Samples by Capillary Electrophoresis

为监测贝类食品的食用安全 ,建立了毛细管电泳 紫外检测法定量测定贝类食品中软骨藻酸的分析方法。结果表明 :软骨藻酸在 0 2～ 5 0 μg mL范围内具有良好的线性关系 ,相关系数r =0 9990 ;RSD =2 5 6 % ;方法检出限为 0 0 34μg g。在 3个水平上对实际样品进行标准添加 ,回收率分别为 96 80 %、97 10 %和 96 85 %。该方法简单、灵敏、高效、成本低 ,对记忆损失性贝类毒素的检测和监控具有重要意义。

A capillary electrophoresis with UV method for determination of domoic acid,a main kind of amnesic shellfish toxin was established.The calibration curve of domoic dacid showed good linearity in the range of 0 2～50 μg/mL with r =0 9990 and RSD =2 56%.The detection limit of the method was 0 0034 μg/g.Spike with domoic acid standard at three levels,the recoveries were 96 80%、97 10% and 96 85% respectively.The method was simple,convenient,sensitive,low cost and suitable for monitoring of amnesic shellfish toxin.