基础减压对慢性压迫性脊髓损伤大鼠ChAT表达的影响

Influence of decompression on the expression of ChAT in chronic spinal cord injury of rats

目的:探讨手术减压对治疗慢性脊髓损伤的作用机理。方法:采用大鼠后路渐进性脊髓压迫动物模型,然后进行手术减压,观察其联合行为评分(CBS),并常规行病理及胆碱乙酰转移酶(ChAT)的免疫组化检测。结果:脊髓损伤后ChAT免疫组化阳性细胞数减少,CBS升高;经手术减压治疗后,ChAT阳性细胞数增多,CBS也改善,减压组与压迫组比较差异具有显著性(P<0.05)。结论:手术减压治疗的作用机制可能是其促进了脊髓运动神经元合成ChAT,从而合成更多的运动神经递质———乙酰胆碱,加速了实验动物行为功能的恢复。

Objective:To study the mechanisms of decompression on chronic spinal cord injury of rats.Method:A model of chronic spinal cord injury was made.After sixty days,the pressure installation were removed.A quantitive analysis was carried out on CBS and positive immunohistochemistry cell of choline acytyltransferase(ChAT).Pathological samples were observed by light microscopic.Result:After spinal cord injury,the numbers of positive neurons of ChAT decreased,CBS raised too.After treated with decompression,positive neurons of ChAT increased in number,and CBS improved too.There were significant difference between the group of decompression and the compression(P<0.05). Conclusion:The treatment of decompression may promote a synthesis of ChAT in injured spinal cord neurons,and enhance the synthesis of motor nerve transmitter (acetylcholine, ACH ),and facilate the behavioral function recovery of experimental animals.