p27^(KIP1)与神经母细胞瘤分化的研究

Study of p27^(KIP1) in Differentiation of Neuroblastoma

目的 探讨p27^(KIPl)在神经母细胞瘤诱导分化及预后的作用。方法用BrdU诱导神经母细胞瘤细胞系TGW的分化,免疫组化法分析神经母细胞瘤分化过程中p27^(KIPl)的表达特点,Western blot法分析p27^(KIPl)的总体表达水平。结果 BrdU引起p27^(KIPl)的表达明显增加,主要位于细胞浆,p27^(KIPl)的表达使神经母细胞瘤细胞株的生长停滞,p27^(KIPl)在诱导分化作用下蛋白质表达的总体水平增加。结论 p27钓(KIPl)的表达与神经母细胞瘤的分化呈正相关,p27^(KIPl)可以抑制神经母细胞瘤的生长并可以成为以促进肿瘤细胞分化的基因治疗方案的基础。

Objective To investigate the expressions of the cell cycle inhibitor p27KIP1 in the process of neuroblastoma differentiation and its roles in treatment and prognosis. Methods A neuroblastoma cell line TGW was used for study. Bromodeoxy uridine (BrdU) was used to induce the differentiation of neuroblastoma; immunohistochemistry was used to study the characteristic expression of p27 ; Western blot analysis was used to determine the overall expression levels of p27 in TGW. Results BrdU could effectively induce the differentiation of TGW cells. Compared with untreated TGW cells, the BrdU treated cells showed significant increase in p27 expression and inhibited tumor cell growth. The location of expressed p27 was mainly confined in cytoplasm; and the overall expression levels of p27 were significantly increased after induction of differentiation in TGW. Conclusion The expressions of p27KIPI positively correlate to the differentiation of neuroblastoma. p27 can inhibit the growth of neuroblastoma and can be a target of gene therapy for induction of differentiation in cancers.