海风藤对β-APP基因表达的影响

Inhibition ofβ-APP gene expression by Caulis Piperis Futokadssurae with RT-PCR

目的:研究海风藤对人类神经母细胞瘤细胞淀粉样前体蛋白(β-APP)基因表达的影响。方法:用细胞培养和逆转录-聚合酶链反应(RT-PCR)观察海风藤对β-APPmRNA的影响。结果:海风藤选择性抑制β-APP基因表达。结论:β-APP与Alzheimer病(AD)的发病关系密切,海风藤选择性抑制β-APP基因达,为其防治AD提供了更加可靠的实验依据。

Objective:To investigate the inhibition of Caulis Piperis Futokadssurae on gene expres-sion ofβ-amyloid precursor protein (β-APP).Methods:Cell culture and RT-PCR were used to determine the level ofβ-APP mRNA.Results:Inhibition of Caulis Piperis Futokadssurae on gene expression ofβ-APP was con firmed in the study.Conclusion:It has been confirmed thatβ-APP is deeply involved in Alzheimer's dis ease.The present study confirmed the inhibitory action of Caulis Piperis Futokadssur ae to the gene expression ofβ-APP,and provided a reliable experimental basis with respect to the prevention and treatment of Alzheimer's disease with Caulis Piperis Futokadssurae