三氧化二砷对HL-60细胞生长的影响

The effect of arsenic trioxide on the growth of HL-60 cell

目的 :观察不同浓度的三氧化二砷 (As2 O3 )对HL 6 0细胞生长的作用。方法 :采用瑞氏染色油镜观察细胞形态 ;四甲基噻唑氮蓝 (MTT)比色法观察As2 O3 对HL 6 0细胞的生长抑制作用。结果 :瑞氏染色显示低浓度的As2 O3 (0 .1μmol/L ,1.0 μmol/L)诱导后 ,细胞呈现部分分化现象 ;而高浓度的As2 O3 (5 .0 μmol/L)诱导后 ,细胞呈现凋亡特征。As2 O3 能明显抑制HL 6 0细胞的生长 ,第 1天半数抑制率 (IC50 )约为 2 0 .0 μmol/L ,第 2天、第 3天IC50 均约为 5 .0 μmol/L。 结论 :As2 O3 能明显抑制HL 6 0细胞的生长 ;低浓度者可诱导HL 6 0细胞部分分化 。

Objective:To observe the effect of arsenic trioxide on the growth of HL 60 cells.Methods:The cell metamorphosis was observed by Wright staining.The growth inhibition was analyzed by tetramethyl thiazole nitroblue(MTT).Results:As 2O 3 at low concentrations can induce partial differentiation of HL 60 cells,while at higher concentration can promote cell apoptosis.As 2O 3 inhibited the growth of HL 60 cells remarkably,the 50% inhibition concidence value of As 2O 3 for HL 60 cells were 20.0 μmol/L,5.0 μmol/L,5.0 μmol/L respectively during 1 to 3 days.Conclusions:As 2O 3 at low concentrations can induce partial differentiation of HL 60 cells,while at high concentrations can promote cell apoptosis.