马尾松松针中木脂素类化学成分的研究

Studies on the lignan chemical constituents from pine needles of Pinus massoniana Lamb.

目的 研究松科 (Pinaceae)植物马尾松 (P .massonianaLamb .)松针 (pineneedles)水煎液中的化学成分。方法 利用DiaionHP 2 0 ,ToyopearlHW 4 0和硅胶等柱色谱技术进行分离纯化 ,根据化合物的理化性质和光谱数据进行结构鉴定。结果 从马尾松针水煎液镇痛作用较强的正丁醇萃取部位分离得到 3个木脂素类化合物 ,其化学结构分别确定为化合物 7(massonianosideD) :(7S ,8R) 4 ,9′ 二羟基 3,3′ 二甲氧基 7,8 二氢苯骈呋喃 1′ 丙醇基新木脂素 9 O α L 鼠李糖苷 ;化合物 8(异落叶松脂素 ) :4 ,4′ ,9,9′ 四羟基 3,3′ 二甲氧基 7 苯基四氢萘木脂素 ;化合物 9(异落叶松脂素 9 O 木糖苷 ) :4 ,4′,9′ 三羟基 3,3′ 二甲氧基 7 苯基四氢萘木脂素 9 O β D 木糖苷。 结论 化合物 7为新化合物 ,化合物 8。

Aim To study the chemical constituents of the pine needles from Pinus massoniana Lamb.. Methods Various chromatographic techniques were used for the separation and purification. The physicochemical properties and spectral data were used to elucidate the structures. Results Three compounds were isolated from the n BuOH fraction of water extracts. The structures were identified as (7 S ,8 R ) 4,9′ dihydroxyl 3,3′ dimethyoxyl 7,8 dihydrobenzofunan 1′ propanolneolignan 9 O α L rhamnopyranoside (massonianoside D, 7), 4,4′,9,9′ tetrahydroxyl 3,3′ dimethyoxyl tetrahydrocyclolignan ((+) isolariciresinol, 8) and 4,4′,9′ trihydroxyl 3,3′ dimethyoxyl tetrahydrocyclolignan 9 O β D xylopyranoside (isolariciresinol 9 O xyloside, 9) on the basis of spectral data (ORD, UV, IR, MS, 1HNMR, 13 CNMR, 1H 1HCOSY, HMQC and HMBC etc .). Conclusion Compound 7 is a new compound, while compounds 8 and 9 were isolated from this plant for the first time.