耐高渗压酵母的甘油发酵研究:ⅡGW-11菌株的发酵条件试验

Study on Glycerol Fermentation of Osmotolerant Yeasts: the Fermentive Conditions Test of Strain GW-11

甘油是国防和民用工业的一种重要原料,日前市场供需矛盾突出。耐高渗酵母发酵法制取甘油是解决这一供求矛盾的有希望的途径,而高产菌株选育是该途径的基础。我组1987年从广西自然环境中筛选获得一株产甘油的菌种GW-11。本文是通过对该菌株的培养基组成及培养条件的试验。以获得最佳发酵条件,并对其甘油生产性状进行考察。结果表明:优化发酵条件与原试验条件相比,该菌发酵甘油产率由原8％提高到14.0％,对糖转化率由原来的40％,提高到46.7％,GW—11是一株具有潜在工业生产价值的耐高渗的甘油发酵生产菌。

Glycerol is an important raw material of national defence and civil industry. Nowaday, the supply of glycerol is unable to meet the demand. Production of glycerol by osmotolerant yeasts provide a promising method to solve this problem. Selecting of high-yield strains of osmotolerant yeasts are the foundation of this mahod. Our laboratory obtained a high-yielding strain GW-11 in 1987 from Guangxi. This article introduce the experiment for obtaining the optimal fermentive conditions by cultivating this strain in different media and different cultivating conditions, the glycerol production characteristics of this strain is also observed. The results demonstrated that: compared with the original, the yield rate of glycerol of the strain in the improved fermentation conditions increase to 14.0％ from 8.0％, and the conversion rate of saceharide increase to 46.7％ from 40.0％. The strain GW-11 has potential value of industrial production of glycerol.