野栽杂交花培育种探讨

Study on Rice Breeding of Anther Culture from the Hybrid Between Wild and Cultivated Rice

采用花药培养技术对普通野生稻白叶枯病广谱抗源RBB16与水稻品种垦系3号等一批杂交后代进行花培育种。接种花药79080个,平均愈伤组织诱导率7.94％,绿苗分化率15.08％,从获得一批野栽杂交后代的花培绿苗中选育出T209-1、14-5两个高产、或高抗白叶枯病的稳定新品系。研究表明野栽杂交花培育种技术是克服野栽杂交后代稳定难,育种周期长与加速野生稻资源利用的有效途径。

Anther culture breeding of progenies of the hybrid between varieties Kenxi No.3 end so on and common wild rice RBB16(O.rufipogon) which has broad spectrum resistance to bacterial blight have been conducted with the techniques of anther culture. Inoculated 79080 anthers, the av- erage induction frequencies of callus and the differentiation frequencies of green plantlets are 7.94% and 15.08% respectively. T209-1, 14-5 two stable lines of higher yield or high resistance to bacterial blight are selected from a lot of pollen plants of the crossed progeny. The results indi- cated the techniques of anther culture on the hybrid between wild and cultivated rice is one of the most effective way to overcome the unsteady of the hybrid progenies, long breeding cycle and to quicken the utilization of wild rice resources.