溴氰菊酯对大鼠脑组织脑源性神经生长因子表达的诱导

Brain-derived neurotrophic factor mRNA expressions in rat brain induced by short-term in vivo exposure to deltamethrin

目的 研究溴氰菊酯 (DM)对中枢神经系统损害的机制。方法 用逆转录 聚合酶链式反应 (RT PCR)、流式细胞分析及免疫组化技术检测溴氰菊酯对大鼠大脑皮层和海马脑源性神经生长因子 (BDNF)表达的影响。结果 大鼠经DM一次大剂量 (DM1 )和多次小剂量 (DM2 )处理后 ,大鼠大脑皮层和海马的BDNFmRNA及蛋白水平均有明显升高。RT PCR结果表明 ,DM1和DM2组大鼠皮层和海马BDNFmRNA的水平分别较对照组高出 48%、56 %、59%、和 54 % ;斑点杂交结果亦显示 ,DM1和DM2组大脑皮层和海马的BDNFmRNA分别是对照组的 1 86 %、1 61 %、1 4 8%和 1 58% ,与RT PCR结果基本一致 ;流式细胞分析结果表明 ,DM1组和DM2组大鼠皮层和海马BDNF蛋白表达量分别较对照组高 53 %、8%和 45 %、46 % ;免疫组化结果表明 ,DM1和DM2组大鼠皮层的蛋白水平分别是对照组的1 2 9%和 1 4 7% ,与流式细胞分析一致 ;而海马主要是DM1组的CA1、DG区和DM2组CA3、DG区明显高于对照组。结论 DM在体内明显诱导大鼠大脑皮层和海马BDNF的表达 。

Objective To elucidate the mechanism of damage on central nervous system (CNS) caused by deltamethrin (DM) Methods The mRNA and protein expressions of brain derived neurotrophic factor (BDNF) in the cerebral cortex and hippocampus of the rats exposed to DM were measured by retro transcription polymerase chain reaction (RT PCR), dot blot, flow cytometry analysis and immunohistochemistry Results After exposure to DM at high dose (DM1, 25 0 mg·kg -1 ·d -1 , ip) once and low dose (DM2, 12 5 mg·kg -1 ·d -1 , ip) for 5 days, the level of BDNF mRNA and protein expression in the cerebral cortex and hippocampus of the rats increased significantly. The levels of BDNF mRNA and protein expression in the cerebral cortex and hippocampus measured by of RT PCR in the rats with DM1 and DM2 were higher than those in the controls by 48% and 56%, and 59% and 54%, respectively. And, those measured by dot blot in the rats with MD1 and MD2 were 186% and 161%, and 148% and 158% of those in the controls, respectively, basically similar to those measured by RT PCR. Flow cytometric analysis showed that the levels of BDNF mRNA and protein expression in the cerebral cortex and hippocampus in the rats with DM1 and DM2 were higher than those in the controls by 53% and 89%, and 45% and 46%, respectively. Immunohistochemical analysis showed that protein expression in the cerebral cortex of the rats with DM1 and DM2 were 129% and 147% of those in the controls, same as the flow cytometric analysis, but those were significantly higher in the hippocampus mainly in the CA1 and DG areas of the rats with MD1 and the CA3 and DG areas of the rats with DM2 Conclusions DM could induce BDNF mRNA and protein expression in the cerebral cortex and hippocampus of the rats, which could play an important role in repairing of nerve damage.