地黄种质资源的离体保存研究

Preservation of Germplasm of Rehmannia glutinosa in Vitro

目的 :探索地黄种质资源的离体保存方法。方法 :应用不同品种的茎尖试管苗为材料 ,接种在装有MS +0 .2BA +0 .0 2NAA的试管中 ,同时将同一品种 (85 5 )的茎尖苗接种在不同培养基 (A .蒸馏水 +10 g·L-1琼脂 ;B .1/2MS +5g·L-1琼脂 ;C .MS +10g·L-1琼脂 ;D .1/2MS +0 .5BA +0 .0 2NAA +10g·L-1琼脂 ;E .MS +0 .5BA +0 .0 2NAA +10 g·L-1琼脂 )上 ,4～ 6℃黑暗保存。结果 :保存 4个月后发现 ,相同培养基中不同品种的死亡率不同 ,土城和邢疙瘩较高 (6 0 % ,73 % ) ,北京 1号和 85 5较低 (9% ,33 % )。 10个月后 ,MS +0 .2BA +0 .0 2NAA上不同品种的保存苗多数死亡。 85 5的保存苗在A号培养基上 ,除 1只试管污染外 ,均处于存活状态 ,其他培养基上的保存苗多数基部褐化死亡。结论 :地黄试管苗对低温的耐受力具有品种特异性 ,A号培养基可实现地黄种质资源的中期保存。

Objective: To establish a method for the germplasm preservation of R. glutinosa. Method: Plantlets of different cultivars obtained by tip culture were inoculated into test tubes with MS medium supplemented with 0.2 mg·L -1 BA and 0.02 mg·L -1 NAA, and preserved at 4-6 ℃ in dark. At the same time, different media (A. distilled water + 10 g·L -1 agar;B. 1/2MS + 5 g·L -1 agar;C. MS + 10 g·L -1 agar;D. 1/2MS + 0.5BA + 0.02NAA + 10 g·L -1 agar;E. MS + 0.5BA + 0.02NAA + 10 g·L -1 agar) were set to conserve plantlets of'85-5' on the same condition. Result: 4 months later, the death rate of 'Xinggeda' was 73%,'Tucheng' 60%, '85-5' 33%, and 'Beijing 1'9%. All of the plantlets of'85-5' in different media kept alive. 10 months later, most of the preserved plantlets browned and wilted except those on medium A. Conclusion: The low temperature endurance of R. glutinosa is cultivar-dependent. Medium A can preserve '85-5' for more than 10 months in vitro.