麝香糖蛋白成分对中性白细胞趋化反应的抑制作用

Effects of Musk Glucoprotein on Chemotaxis of Polymorphonuclear Leukocytes in vivo and in vitro

目的 :观察麝香糖蛋白成分 (麝香 1)对中性白细胞趋化反应的影响。方法 :体内采用羧甲基纤维素诱导大鼠腹腔内中性白细胞趋化反应模型。体外采用Boyden隔室法测定兔中性白细胞趋化反应。以Fura 2标记荧光法测定细胞内游离钙水平。结果 :皮下注射麝香水提物 5 ,2 0 ,80mg·kg-1,显著抑制羧甲基纤维素诱导的大鼠腹腔中性白细胞趋化反应 ,抑制率分别为 5 8.6 % ,6 5 .1%和 73 .0 % ;终浓度为 1,10 ,10 0 μg·mL-1麝香 1,显著抑制fMLPP诱导的兔中性白细胞的趋化反应 ,抑制率分别为 5 7.9% ,6 4.7%和 77.8% ;终浓度为 1,10 ,10 0 μg·mL-1的麝香 1,明显抑制刺激剂引起的中性白细胞胞内游离钙水平的升高 ,抑制率分别为 16 .3% ,2 4.7%和 37.8%。结论 :麝香糖蛋白成分对中性白细胞趋化反应有显著抑制作用 ,是其抗炎作用机制之一。

Objective: To investigate the effects of Musk glucoprotein on chemotaxis of Polymorphonuclear leukocytes(PMN). Method: The chemotaxis of PMN in abdominal cavity in rat induced by carboxymethyl cellulose(CMC) was used as an in vivo animal model and in in vitro it was evaluated by Boyden chamber. The concentration of cytosolic free Ca 2+ was quantitated with the fluorescent Ca 2+ indicator Fura-2. Result: The water extract of Musk at dose of 5,20,80mg·kg -1 (sc)significantly inhibited the chemotaxis of PMN in rat; Musk-1 at concentration of 1-100 μg·mL -1 can significantly inhibit the chemotaxis of rabbit PMN in vitro ; Musk-1 at concentration of 1-100 μg·mL -1 can significantly inhibit the increase of cytosolic Ca 2+ concentration in PMN of rat. Conclusion: Part of mechanisms underlying antiinflammatory action of Musk is to inhibit the chemotaxis of PMN.