葛根素对血管内皮损伤后血小板活化状态及内膜厚度的影响

Effects of Puerarin(葛根素) on platelet activation and intimal thickening after vascular balloon injury in rats

目的 :研究球囊损伤血管内皮后内膜增生的过程、血小板活化水平及中药葛根素对它们的影响。方法 :球囊剥脱大鼠主动脉内皮 ,并随机分为对照组、手术组和葛根素治疗组。分别在术后 3、7、14和 2 8d取主动脉 ,通过组织学检查和放射免疫技术检测内膜增生的情况、血小板表面 GMP 140的数目以及葛根素( 5 0 m g· kg- 1 · d- 1 )腹腔注射后上述指标的变化。结果 :1GMP 140于术后 3 d明显升高 ,术后 7d开始下降。 2术后 3 d已有血管平滑肌细胞 ( VSMC)的移行和增殖 ;7d内膜开始增生 ;2 8d VSMC的增殖减弱 ,但细胞外基质增加 ,内膜继续增生。3使用葛根素后血小板表面 GMP 140的数目和 VSMC的移行减少 ,但内膜增生程度无明显改变。结论 :血管内皮损伤后血小板活化水平升高 ,VSMC移行、增殖及细胞外基质增加 ,葛根素能抑制血小板活化及 VSMC的移行 ,但对内膜增生的程度无明显影响。

Objective:To investigate the process of neointimal formation,the level of platelet activation,and the effects of Puerarin(葛根素) on them after vascular balloon injury in rats.Methods:Rat models of aortic endothelial denudation by balloon catheter were established.Rats were randomly allocated into three groups.Group 1 (n=24) served as controls.Group 2 (n=24) were given vascular balloon injury.Group 3 (n=24) were given Puerarin (50 mg·kg -1·d -1) from 1 day before injury to 28 days after injury.The number of platelet αgranule membrane protein140(GMP140) and the process of intimal thickening were investigated at day 3,7,14 and 28 after injury by radioimmunological technique and histological method, respectively. Results:①The number of platelet GMP140 were higher obviously at day 3 and began to decrease at day 7 after injury.②The migration and proliferation of vascular smooth muscle cells(VSMC) had existed at day 3 after balloon injury.The thickening of intima had begun at day 7 after injury,and they were more significant at day 14.The proliferation of VSMC decreased at day 28,but extracellular matrix increased and the intimal thickening continued.③ When given Puerarin,the number of platelet GMP140 and the migration of VSMC reduced,but the intimal thickening had no significant variation.Conclusion:The number of platelet GMP140 increase in the process of intimal thickening after balloon injury.Puerarin inhibits the activation of platelet and migration of VSMC,but has no obvious effect on intimal thickening.