丹参对烧伤大鼠肠黏膜上皮细胞线粒体功能的影响

Effect of salvia miltiorrhiza(丹参) on mitochondria function of intestinal mucosa in burnt rats

目的 :探讨丹参对烧伤大鼠肠黏膜上皮细胞线粒体呼吸功能及氧自由基的影响。方法 :96只 SD大鼠按随机数字表法分为对照组 ( A)、烧伤组 ( B)、治疗组 ( C)。B组和 C组造成 2 0 %总体表面积 度烧伤。在伤后1、2、6 h,动态检测肠黏膜上皮细胞线粒体内细胞色素 aa3、细胞色素 C、能荷和超氧化物歧化酶 ( SOD)水平。结果 :1伤后 2 h内 ,B、C组肠黏膜上皮细胞线粒体内细胞色素 aa3水平无显著降低 ( P均 >0 .0 5 )。伤后 6 h,B、C组肠黏膜上皮细胞线粒体内细胞色素 aa3水平均显著低于 A组 ( P均 <0 .0 5 ) ;同时 C组显著高于 B组( P<0 .0 5 )。2伤后 1h始 ,大鼠肠黏膜上皮细胞线粒体内细胞色素 C水平、能荷和 SOD活力下降 ;C组显著高于 B组 ( P均 <0 .0 5 )。结论 :丹参能显著提高细胞色素 aa3、细胞色素 C、能荷和 SOD水平 ;明显改善大鼠烧伤后肠黏膜上皮细胞线粒体呼吸功能及明显减少氧自由基的产生。

Objective:To study the effect of salvia miltiorrhiza(丹参) on mitochondria function of intestinal mucosa and oxygen radicals in burnt rats.Methods:Ninetysix SpregueDawley(SD) rats were randomly divided into control group(A),burn group (B),treatment group(C).Rats in B and C groups were subjected to a 20% total body surface area (TBSA) fullthickness thermal injury.At different time points,the dynamic levels of cytochrome aa3,cytochrome C,energy production and superoxide dismutase(SOD) in mitochondria of intestinal mucosa were determined.Results:①Two hours after burn,the levels of cytochrome aa3 in B and C groups did not change obviously(both P>0.05).Six hours after burn,they were much lower than that in A group,and the level of cytochrome aa3 in C group were obviously higher than those in B group (all P<0.05).②From 1 hour after burn,the levels of cytochrome C,energy production and SOD in B and C group were decreased,and those in C group were obviously higher than those in B group (all P<0.05).Conclusion:Salvia miltiorrhiza could enhance the levels of cytochrome aa3,cytochrome C,energy production and SOD,which could obviously protect mitochondria function of intestinal mucosa and obviously reduce the oxygen radicals production.