中风星蒌通腑胶囊对急性脑缺血大鼠脑组织中兴奋性氨基酸的影响

Effects of Zhongfeng Xinglou Tongfu capsule(中风星蒌通腑胶囊) on excitatory amino acid in cerebral tissues of rats with acute cerebral ischemia

目的 :探讨中风星蒌通腑胶囊治疗急性脑缺血的作用机制及与兴奋性氨基酸 ( EAA)的关系。方法 :以急性全脑缺血大鼠为模型 ,用华佗再造丸作为阳性对照 ,将 72只普通级 Wistar大鼠随机分为假手术组、模型组、华佗再造丸组及中风星蒌通腑胶囊高、中、低剂量组 ,分别灌胃并取材后 ,测定脑组织中谷氨酸 ( Glu)、天门冬氨酸 ( Asp)含量。结果 :急性脑缺血损伤后 ,模型组大鼠脑组织中 Glu、Asp含量明显升高。在降低脑组织Glu、Asp方面 ,中风星蒌通腑胶囊高、中、低剂量组均效果显著 ,与模型组比较 ,有显著差异 ( P <0 .0 5或P<0 .0 1) ;华佗再造丸组对 Glu、Asp含量影响不明显 ( P均 >0 .0 5 )。结论 :中风星蒌通腑胶囊具有降低急性脑缺血大鼠脑组织中 EAA的作用 ,而且在这方面的作用优于华佗再造丸。

Objective:To observe the curative effect of Zhongfeng Xinglou Tongfu capsule(ZFXLTFC,中风星蒌通腑胶囊) on rats with acute cerebral ischemia,and the correlativity with excitatory amino acid(EAA) in cerebral tissues.Methods:The rats with acute wholecerebral ischemia were used as model,Huatuo Zaizao pill(HTZZP,华佗再造丸) was used as positive contrast.Seventytwo common Wistar rats were randomly divided into shamoperation group,model group,HTZZP group and highdose,middledose, lowdose groups of ZFXLTFC.ZFXLTFC and HTZZP were given via gastric gavage respectively.Stomach tissues were obstained,the contents of glutamate(Glu) and asparate(Asp) in the cerebric tissues were measured. Results:The contents of Glu and Asp in the cerebral tissues of model group were increased significantly than shamoperation group(both P<0.01) after acute cerebral ischemia.The levels of Glu and Asp were reduced in the three ZFXLTFC groups (P<0.05 or P<0.01 ).The high levels of Glu and Asp didn't change remarkablely in the HTZZP group(both P>0.05).Conclusion:ZFXLTFC could reduce the contents of EAA in cerebral tissues of rats with acute cerebral ischemic.Its effect is better than that of HTZZP.