HPLC测定消食止泻散中橙皮苷的含量

Determination of Hesperidin in Xiaoshizhixie Power by HPLC

目的 建立消食止泻散中橙皮苷的含量测定方法。方法 超声提取 ,HPL C测定消食止泻散中橙皮苷的含量。 C1 8柱 ,乙腈 -水 -磷酸 (180∶ 82 0∶ 0 .1)为流动相 ,检测波长为 2 83nm,柱温 :2 8℃。结果 橙皮苷含量测定的线性范围为 0 .197μg～ 1.182μg,r=0 .9999,n=6 ,平均加样回收率为 99.8 ,RSD为 1.3%。结论 本方法准确 ,简便 ,快速 ,可用于消食止泻散的质量控制。

Objective To establish the method for determining hesperidin in Xiaoshizhixie Power by HPLC.Methods Samples were extracted by ultrasonic wave;HPLC was used to determine the content of hesperidin in samples on Kromasil C 18 column with acetonitrile water phosphoric acid(180∶820∶0.1) as the mobile phase.The detection wavelength was 283 nm.The column temperature was 28 ℃.Results The linear range of hesperidin was 0.197 μg～1.182 μg(r=0.999 9,n=6).The average recovery was 99.8%,RSD was 1.3%.Conclusion The method is accurate,simple and quick.It can be used for quality control of Xiaoshizhixie Power.