MK-801和硫酸镁对新生大鼠缺氧缺血性脑病的保护作用

Neuroprotection of MK-801 and magnesium sulfate in 7-day rats with hypoxic ischemic encephalathy(HIE)

[目的 ]研究在缺氧缺血性脑病时MK - 80 1和硫酸镁对碱性成纤维细胞生长因子 (bFGF)的影响。 [方法 ]选用新生 7日大鼠制成新生儿缺氧缺血性脑病动物模型 ,共分为 4组。 (1)正常组 :分离左侧颈总动脉后在动脉下置线 ,不结扎亦不低氧。 (2 )缺氧缺血处理组 :模型后立即腹腔注射生理盐水 0 .1mL/只。 (3)MK - 80 1处理组 :模型后立即腹腔注射MK - 80 11mg·kg- 1。 (4 )硫酸镁处理组 :模型后立即腹腔注射硫酸镁 30 0mg·kg- 1。上述各组每组 5只。均在模型后 2 4h断头处死 ,采用免疫组化方法检测bFGF。 [结果 ]缺氧缺血侧 (左侧 )大脑皮层bFGF阳性神经细胞数显著多于 (P <0 .0 1)正常组 ;MK - 80 1组及硫酸镁组。 [结论 ]大脑缺氧缺血后MK - 80 1及硫酸镁可上调bFGF ,具有保护脑组织作用。

To study the effect of MK-801 and magnesium sulfate on bFGF treating 7-old-day rats with HIE. The model of 7-old-day rats with HIE was established.This study included four groups:normal group(no hypoxia and ischemia ); HIE group(normal saline 0.1 mL/kg); MK-801 treatment group(1 mg/kg after HIE);sulfate magnate treatment group(300mg·kg -1 after HIE) . Every group had 5 rats,all rats were killed 24 h after HIE. The 6FGF positive neural cells of HIE group were more than those of the normal group ( P <0.01), and those of MK-801 and magnesium sulfate groups were more then that of HIE group. [Conclusion] MK-801 and magnesium sulfate may behave protective effect on 7-old-day rat with HIE by improving bFGF.