小檗碱、齐墩果酸和大蒜新素对糖代谢作用的体外研究

In Vitro Research on the Effects of Berberine,Oleanolic Acid and Allitridi on Glucose Metabolism

目的 研究小檗碱、齐墩果酸和大蒜新素能否通过直接作用于肝细胞或刺激胰岛素分泌而产生降糖作用。方法 采用与人肝细胞表型相似的HepG2细胞 ,检测 2 4h培养液中葡萄糖的消耗量。采用能稳定分泌胰岛素的 βTC3细胞检测胰岛素的释放量。用MTT法监测细胞增殖的情况。结果 小檗碱可使HepG2细胞的葡萄糖消耗量增加 6 9.1% (P <0 0 0 0 1) ,齐墩果酸和大蒜新素可使糖耗量轻度增高。齐墩果酸还使 βTC3细胞的胰岛素分泌量减少 2 5 %～ 2 9% (P <0 .0 1) ,小檗碱和大蒜新素对胰岛素分泌没有明显的影响。

Objective To study the effects of berberine, oleanolic acid and allitridi to see whether they can exert, either by directly acting on hepatocytes or by activating the secretion of insulin, the effect of lowering the level of glucose. Methods HepG2 cells, the phenotype of which is similar to that of the human hepatocytes, were used to detect the 24 hour consumption of glucose in the culture fluid; βTC3 cells, which can stably secrete insulin, were used to detect the release of insulin; and MTT assay was used to monitor the proliferation of the cells. Results Berberine could increase the glucose consumption of HepG2 cells by 69 1% ( P <0 0001), and both oleanolic acid and allitridi could slightly elevate the glucose consumption; oleanolic acid could decrease the insulin release of βTC3 cells by 25%～29% ( P <0 01) while both berberine and allitridi did not show marked effects on the insulin release. Conclusion Berberine can exert a remarkable effect for lowering the level of glucose by acting on hepatocytes.