探讨人体泰素帝药物血清诱导肺癌细胞凋亡的分子机制

Study on molecular mechanism about inhibiting lung cancer cells by docetaxel serums from patients

目的 探讨肿瘤患者药物血清对诱导人肺腺癌细胞系 (SPC A 1)细胞凋亡的分子机制。方法 经采用AnnexinⅤ FITC标记的细胞流式仪、RT PCR、免疫组化以及Western印迹法分别检测泰素帝化疗后 1h及 4h肿瘤患者外周血药物血清诱导之人肺癌细胞SPCA 1中bcl 2、Bax及Caspase 3基因表达水平以及Caspase 3酶原激活等与凋亡相关的分子变异。 结果 ( 1)经AnnexinⅤ FITC标记结合细胞流式仪法检测发现含泰素帝药物血清可诱导SPC A 1细胞凋亡。 ( 2 )泰素帝化疗后患者 1小时药物血清可导致SPC A 1细胞中bcl 2基因表达水平下降 ,但bax基因表达维持不变 ,促使bcl 2 /bax之间基因表达比值明显下降。 ( 3)SPCA 1细胞凋亡时未见caspase 3基因m RNA表达水平改变 ,但出现Caspase 3酶原活化。 结论 ( 1)含泰素帝药物人体血清可诱导体外培养人肺腺癌细胞系SPC A 1细胞凋亡。 ( 2 )药物血清诱导肿瘤细胞的凋亡可能是通过bcl 2 /bax基因表达比值降低的分子途径。 ( 3)药物血清导致肿瘤细胞凋亡程序可能是依赖于激活Caspase 3酶原途径而与该基因表达水平无关。

Objective To investigate the molecular events about inhibiting human lung adenocarcinoma cells (SPC-A-1 cell line) by using drug serums from cancer patients in which contained Docetaxel. Methods The apoptosis of SPC-A-1 cells was determined by FCM with AnnexinⅤ-FITC labeling. The expressions of bcl-2, bax and caspase-3 genes were examined by RT-PCR and immunohistochemistry respectively, as well as the enzyme activation of caspase-3 by Western blot. Results (1) A relative higher proportion of apoptosis was observed in SPC-A-1 cells treated with drug serum from patients one hour after the administration of Docetaxel. (2) The expression of bcl-2 gene was obviously lower in the group of cells treated with 1 hour drug serum, while the expression of bax gene was consistent in both treated or untreated groups. The expression rate of bcl-2 to bax gene was decreased significantly in the group of cells treated with 1 hour drug serum than other groups. (3) The m-RNA expression of caspase-3 was not changed in the treated groups, while the activation of caspase-3 was detected by Western blot. Conclusion (1) Drug serum containing Docetaxel can induce apoptosis of SPC-A-1 cells. (2) The expression of bcl-2 gene is involved in the apoptosis of lung cancer cell induced by drug serum in vitro. (3) The molecular event of cell apoptosis may be dependent on the activation of Caspase-3 enzyme rather than the expression of caspase-3 gene.