宽叶缬草对高脂血症大鼠肾小球α-平滑肌肌动蛋白表达的影响

Effect of Valeriana Officinalis Var Latifolia on the Expression of Glomerular α-Smooth Muscle Actin in Hyperlipidemic Rats

目的 :探讨宽叶缬草对高脂血症大鼠肾小球α 平滑肌肌动蛋白 (α SMA)表达的影响及其意义。方法 :将Wistar大鼠 2 4只随机分为高脂血症组 (高脂组 )、宽叶缬草组 (缬草组 )和正常组各 8只 ,高脂组和缬草组饲喂由含 4%胆固醇和 1%胆酸钠组成的高脂饲料 ,正常组饲喂普通饲料 ,缬草组同时给予宽叶缬草提取物 (缬草挥发油剂量 2 5mg·kg 1 ·d 1 )灌胃 16周。观察大鼠血脂、尿蛋白、血肌酐和肾组织病理改变 ,并采用免疫组化技术检测肾小球α SMA表达。结果 :血清总胆固醇及低密度脂蛋白浓度高脂组大鼠显著高于缬草组和正常组 (P <0 .0 1) ,缬草组高于正常组 (P <0 .0 1) ;高脂组尿蛋白和血肌酐较缬草组和正常组明显增高 (P <0 .0 1) ,缬草组尿蛋白高于正常组 (P <0 .0 5 ) ;肾病理和免疫组化证实宽叶缬草在降脂同时 ,能减轻肾小球系膜病变和细胞外基质产生 ,降低肾小球α SMA表达。结论 :宽叶缬草有降低总胆固醇、低密度脂蛋白、尿蛋白和血肌酐的作用 ,并可以保护肾功能 。

Objective:To approach the effect of Valeriana officinalis var latifolia (VOL) on the expression of glomerular α smooth muscle actin (α SMA) in hyperlipidemic rats and its possible significance. Methods:24 Wistar rats were randomly divided into the hyperlipid group, VOL group and control group, with 8 rats in each group. Rats in the hyperlipid and VOL groups were fed with a chow containing 4% of cholesterol and 1% of sodium cholate, while those in the control group, with conventional chow. In addition, rats of the VOL group were given each VOL extract (containing VOL volatile oil in a dosage of 25 mg·kg 1 ·d 1 ) administered through gastric tubes for 16 consecutive weeks. Serum lipid, urinary protein excretion(UPE), serum creatinine (Scr), mesangial cell index and mesangial matrix index were kept under observation. Expression of glomerular α SMA was detected with the immunohistochemical technique. Results:VOL was found to decrease the serum levels of total cholesterol, low density lipoprotein, UPE and Scr. Light microscopy and immunohistochemical staining revealed that the mesangial cell index and mesangial matrix index were also significantly reduced in rats of the VOL group, accompanied with a decreased expression of glomerular α SMA. Conclusion:VOL was shown to have the effect of reducing serum lipid and protecting renal function, which might be attributed to the inhibition of glomerular mesangial cells undergoing phenotypic change.