模拟推拉动作对大鼠脑皮层神经元损伤的实验研究

The experimental study on injury of cortical neurons in rats by simulated push-pull maneuver

目的 探讨大鼠经不同强度推拉动作暴露后，脑皮质神经元损伤程度和时相变化规律。方法 健康雄性Wistar大鼠35只，随机分为7组，每组5只；除对照组外，各组大鼠于暴露后0.5,6,24h进行灌注固定，取大脑半球，切片后分别进行HE染色和尼氏染色，镜下观察结果。结果 实验B2，B3组可见大鼠额叶皮质有一定数量的神经元形态发生改变。但实验B3组损伤程度相对较轻，其他各组无明显变化。结论 低强度推拉动作不会引起明显的大鼠脑皮质神经元损伤，而高强度推拉动作可对大脑造成较大损害。

Objective To investigate the injury of cortical neurons and regularity of phase change in rats after they were exposed to push pull maneuver of various degrees.Methods Thirty five healthy male Wistar rats were randomly divided into 7 groups and there were 5 rats in each group.Except the rats in the control group,the rats in other groups were perfused and fixed respectively after 0.5 hour,6 hour and 24 hour exposure and then the brain hemispheric tissues were taken and sectioned for HE staining and Ni...