γ-干扰素与维拉帕米联合逆转人肺癌细胞系的多药耐药

Combined Use of VPL、INF-γand DDP to Revert the Multidrug Resistance of Lung Cancer Cell Line

目的 探讨逆转人肺癌耐药系HTB - 5 6R耐药性的可行性。方法 采用MTT比色法研究钙通道阻滞剂维拉帕米和人类重组γ -干扰素对DDP细胞毒性在人类肺癌细胞系HTB - 5 6R上的的影响。结果 γ -干扰素与维拉帕米能部分恢复HTB - 5 6R对顺铂 (DDP)的敏感性。当维拉帕米浓度 >4mg/L时 ,能明显增加DDP的细胞毒性 ,耐药株的抑制率 >19.0 % (P <0 .0 5 )。γ -干扰素浓度 >4.0× 10 6 U/L时 ,也能达到此效果 ,细胞抑制率 >3 6.9% (P <0 .0 5 )。联合两药比单独使用有更好的效果 ,维拉帕米为 2mg/L、γ -干扰素为 2 .0× 10 6 U/L时 ,细胞抑制率就达 3 6.9% (P <0 .0 1)。不论单独使用还是联合使用逆转效果均呈剂量效应。结论 γ -干扰素与维拉帕米能逆转HTB - 5 6R的耐药性 ,而且联合使用有更好的效果。

Objective To probe into the possibility of reversion of the drug resistance in HTB-56R. Methods The effect of VPL, an antagonists to calcium channel and INF-γ(recombinant human γ-interferon) on the cytotoxicity of DDP in human lung cancer cell line HTB-56R were studied in vitro by MTT colorimetric assay. Results The results showed that drug sensitivty to DDP was partly reestablished by VPL or INF-γ. VPL at concertration above 4mg/L could significantly enhance the cytotoxicity of DDP, the lung cancer cell line inhibition rate >19.0% ( P <0.05).INF-γ at concertration above 4.0×10 6U/L could also significantly enhance the cytotoxicity of DDP, the inhibition rate >36.9% ( P <0.05). Combinatin of VPL and INF-γ had better result than either of the two alone, the dose of VPL at 2mg/L 、INF-γ at 2.0×10 6U/L, the inhibition rate being 36.9% ( P <0.01). The extent of enhancement is correspondingly increased as the dose is increased. Conclusion VPL and INF-γ can revert the multidrug resistance of HTB-56R. Combination of VPL and INF-γ have better result than either of the two alone.