胃癌组织中树突状细胞和自然杀伤细胞浸润程度与临床病理特征分析

Investigation of the relationship between tumor-infiltrating of dendritic cells and natural killed cells and the clinicopathological characteristics in gastric carcinoma

目的探讨胃癌组织内浸润的树突状细胞 (TIDC)和自然杀伤 (NK)细胞与胃癌临床病理特征的关系。方法以32例手术切除的胃癌标本为研究对象 ,利用S 10 0和CD5 7单克隆抗体分别标记胃癌组织中浸润的TIDC和NK细胞 ,分别统计TIDC和NK细胞数量 ,并以此分为高浸润组和低浸润组。结果淋巴结转移阳性患者TIDC和NK细胞低度浸润的比例均较高 ,分别为 84 .6 %与 84 .6 % ;远处转移患者 10 0 %有TIDC低度浸润 ,综合分析TIDC和NK细胞与胃癌临床病理特征 ,提示TIDC和NK细胞其表达与淋巴结转移呈负相关 (P <0 .0 5 ) ,而与其他临床病理特征无关。结论胃癌瘤内TIDC和NK细胞高度浸润可能会阻止胃癌淋巴结转移 ,TIDC浸润还与远处转移相关 ,因而统计瘤内TIDC和NK数量可能有助于预测胃癌的预后。

Objective To explore the relationship between the tumor-infiltration of dendritic cells(TIDC) and natural killed cells(NK) and the clinicopathological characteristics in gastric carcinoma. Methods 32 resected gastric carcinoma specimens were selected as subjects. S-100 and CD57 antibody were used to stain TIDC and NK infiltrated in gastric carcinoma tissue. The numbers of TIDC and NK cells were counted, respectively, and divided into high and low infiltration groups. Results The rates of TIDC and NK cells infiltration in the patients with lymph node metastasis were significantly higher(84.6%, 84.6%; P<0.05 ). All patients with the distant metastasis had high level of TIDC(100%). Analysis of the combination of TIDC and NK cells and the clinicopathological characteristics showed that the co-expression of TIDC and NK cells was negatively related with the lymph node metastasis(P<0.05). Results Because the high infiltration of TIDC and NK cells in gastric cancer might play a role in antitumor metastasis, the count of TIDC and NK cells might be helpful for predicting the prognosis of gastric carcinoma.