藏药五根散对缺血性脑损伤大鼠新皮质与海马中内皮素含量变化的影响

Experimental Study of Tibetan Medicine on Endothelin Level in Cerebral Cortex and Hippocampus During Ischemia and Ischemically Reperfused Rat Brain

本文观察急性缺血性脑损伤饲喂藏药五根散后大鼠新皮质和海马中内皮素(ET)含量变化,旨在寻找藏药防治脑损伤的可行性。方法是用夹闭大鼠双侧颈总动脉制作急性脑缺血动物模型,然后给该大鼠喂服藏药,以放射免疫法测定脑组织(新皮质和海马)中ET含量,并与喂水组及正常组进行对比观察。结果表明:缺血性脑损伤后大鼠脑皮质与海马中ET含量明显升高,喂服藏药治疗组的ET含量比喂水组的明显下降(P<0.05)。表明缺血性脑损伤后大鼠新皮质及海马中的ET含量增加,而藏药可抑制ET合成使其含量下降,具有保护脑组织作用。

To investigate the effect of Thibetan Medicine therapy on ischemic brain injury by means of endothelin concentration in cerebral cortex and hippocampus with ischemic rat brain the models of cerebral ischemia and ischemie reperfusion were made by means of clipping bilateral common carotid artery in 36 female Wistar rats.These rats were randomly divided into ischemic group (n=18)and ischemic reperfusion group(n=18).Each group was subdivided into treated group admimistered Tibetan Medicine(n=10)and untreated group (n=18).The changes of endothelin concentration in cerebral cortex and hippocampus were observed with radioimmunologic technique after 50 days for operation.It was found that the level of endothelin in untreated groups was markedly increased as compared with treated groups (P<0.05).The changes of endothelin in treated groups were not significant as compared with the sham operation group(P>0.05).The decrease of endothelin of cerebral cortex and hippocampus after Tibetan Medicine cure may be one of the protective mechanism during Tibetan Medicine on brain injury.